PA5-80470

antibody from Invitrogen Antibodies

Targeting: CETN2 CALT, CEN2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80470

Provider

Invitrogen Antibodies

Product name

Centrin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human CETN2/Centrin 2.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Centrin 2 Polyclonal Antibody (Product # PA5-80470) at 1:500 dilution. Lane A: A549 Whole Cell Lysate, Lane B: HT29 Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of His-Centrin 2 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Centrin 2 Polyclonal Antibody (Product # PA5-80470, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of His-Centrin 2 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Centrin 2 Polyclonal Antibody (Product # PA5-80470, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of His-Centrin 2 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Centrin 2 Polyclonal Antibody (Product # PA5-80470, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.