PA5-92443

antibody from Invitrogen Antibodies

Targeting: CETN2 CALT, CEN2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92443

Provider

Invitrogen Antibodies

Product name

Centrin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MASNFKKANM ASSSQRKRMS PKPELTEEQK QEIREAFDLF DADGTGTIDV KELKVAMRAL GFEPKKEEIK KMISEIDKEG TGKMNFGDFL TVMTQKMSEK DTKEEILKAF KLFDDDETGK ISFKNLKRVA KELGENLTDE ELQEMIDEAD RDGDGEVSEQ EFLRIMKKTS LY; Positive Samples: U-251MG, SKOV3, HeLa, HepG2, A-549, Mouse brain, Mouse kidney, Rat brain; Cellular Location: Cytoplasm, Nucleus, centriole, centrosome, cytoskeleton, microtubule organizing center

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.47 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Centrin 2 in extracts of various cell lines using Centrin 2 Polyclonal Antibody (Product # PA5-92443) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Centrin 2 in extracts of various cell lines using Centrin 2 Polyclonal Antibody (Product # PA5-92443) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of A549 cells using CETN2 Polyclonal antibody (Product # PA5-92443).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Centrin 2 was performed in A549 cells using Centrin 2 Polyclonal Antibody (Product # PA5-92443). Blue: DAPI for nuclear staining.