Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA501760 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501760, RRID:AB_11141766
- Product name
- Anti-PIK3AP1 mouse monoclonal antibody, clone OTI7B12 (formerly 7B12)
- Antibody type
- Monoclonal
- Description
- Anti-PIK3AP1 mouse monoclonal antibody, clone OTI7B12 (formerly 7B12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PIK3AP1
- Isotype
- IgG
- Antibody clone number
- OTI7B12
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (RC214125, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PIK3AP1 mouse monoclonal antibody (TA501760) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIK3AP1(RC214125).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PIK3AP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501760, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC214125 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PIK3AP1 antibody(TA501760), and then analyzed by flow cytometry.
- Validation comment
- FC
