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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- BS-13513R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR110 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human A549 and A431 cell lysates probed with GPR110 Polyclonal Antibody, unconjugated (bs-13513R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded human liver carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GPR110 Polyclonal Antibody, Unconjugated (bs-13513R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney cells probed with GPR110 Polyclonal Antibody, Unconjugated (bs-13513R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
