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- Antibody Data
- Antigen structure
- References [2]
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- Immunocytochemistry [3]
- Other assay [1]
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- Product number
- PA5-21775 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Coronin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.
Novel Potential Biomarkers Associated With Epithelial to Mesenchymal Transition and Bladder Cancer Prognosis Identified by Integrated Bioinformatic Analysis.
Galloni C, Carra D, Abella JVG, Kjær S, Singaravelu P, Barry DJ, Kogata N, Guérin C, Blanchoin L, Way M
The Journal of cell biology 2021 Aug 2;220(8)
The Journal of cell biology 2021 Aug 2;220(8)
Novel Potential Biomarkers Associated With Epithelial to Mesenchymal Transition and Bladder Cancer Prognosis Identified by Integrated Bioinformatic Analysis.
Wang C, Yang Y, Yin L, Wei N, Hong T, Sun Z, Yao J, Li Z, Liu T
Frontiers in oncology 2020;10:931
Frontiers in oncology 2020;10:931
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Coronin 3 in methanol-fixed HeLa cells using a Coronin 3 polyclonal antibody (Product # PA5-21775) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C antibody (Product # PA5-21775) at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C antibody (Product # PA5-21775) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8. Coronin 1C recruits MICAL2 to vaccinia-induced actin tails. (A) Image stills from Video 8 and Video 9 showing coronin 1C is required to recruit GFP-MICAL2 (green) to vaccinia-induced actin tails visualized with LifeAct-iRFP (magenta). Scale bars = 5 um (main image) and 2.5 um (insert). The immunoblot shows the level of coronin 1C in HeLa cells treated with coronin 1C siRNA, while the graph shows the percentage of actin tails recruiting GFP-MICAL2 following coronin 1C knockdown. The number of cells and actin tails analyzed is indicated. Student's t test was used to determine statistical significance for n = 4 independent experiments. (B) Immunoblot analysis of GFP-Trap pulldowns on HeLa cells stably expressing the indicated GFP-tagged protein demonstrates coronin 1C (Coro1C) associates with MICAL2, but not MICAL1.
