PA5-85446

antibody from Invitrogen Antibodies

Targeting: EXOSC5 hRrp46p, MGC12901, p12B, RRP41B, RRP46, Rrp46p

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85446

Provider

Invitrogen Antibodies

Product name

EXOSC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution. Predicted reactivity: Human (99%), Mouse (91%), Rat (91%), Pig (90%). Positive Control: HeLa, HeLa nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.35 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EXOSC5 in HeLa whole cell and nuclear extracts using EXOSC5 polyclonal antibody (Product # PA5-85446) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of EXOSC5 in 4% paraformaldehyde-fixed HeLa cells using EXOSC5 polyclonal antibody (Product # PA5-85446) at a dilution of 1:500. Sample was then incubated with phalloidin and Hoechst secondary antibody at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EXOSC5 in paraffin-embedded BT483 xenograft using EXOSC5 polyclonal antibody (Product # PA5-85446) at a dilution of 1:500.