PA5-27760

antibody from Invitrogen Antibodies

Targeting: UGP2 SVUGP2, UGP1, UGPP1

Antibody data

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Validation data

UGP2 protein structure - PA5-27760 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using UGP2 Polyclonal Antibody (Product # PA5-27760). Sample (30 µg of whole cell lysate). Lane A: H1299 . 7.5% SDS PAGE. UGP2 antibody . UGP2 Polyclonal Antibody (Product # PA5-27760) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of UGP2 was achieved by transfecting Hep G2 cells with UGP2 specific siRNA (Silencer® select Product # S14645). Western blot analysis (Fig. a) was performed using whole cell extracts from the UGP2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with UGP2 Polyclonal Antibody (Product # PA5-27760, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to UGP2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-UGP2 Polyclonal Antibody (Product # PA5-27760) and a 56 kDa band corresponding to UGP2 was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), A-431 (Lane 3), HeLa (Lane 4), HEK-293 (Lane 5), tissue extracts of Mouse Liver (Lane 6), Rat Liver (Lane 7), Mouse White Adipose (Lane 8), Rat White Adipose (Lane 9), Mouse Lung (Lane 10) and Rat Lung (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).