Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-60975 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WAVE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DSASSPSPSF SEDNLPPPPA EFSYPVDNQR GSGLAGPKRS SVVSPSHPPP AP Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 90%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of WAVE2 was achieved by transfecting MCF 10A with WAVE2 specific siRNAs (Silencer® select Product # s19803, s19802). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the WAVE2 knockdown cells (lane 2) and non-specific scrambled siRNA transfected cells (lane 1). The blot was probed with WAVE2 Polyclonal Antibody (Product # PA5-60975, 0.7 ug/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to WAVE2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-WAVE2 Polyclonal Antibody (Product # PA5-60975) and a 64 kDa band corresponding to WAVE2 was observed across all the cell lines tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), Caco-2 (Lane 2), C2C12 (Lane 3), MCF 10A (Lane 4), MDA-MB-231 (Lane 5), K-562 (Lane 6) and U-2 OS (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.7 ug/ml) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of WAVE2 in human cell line U-2 OS shows positivity in plasma membrane. Samples were probed using a WAVE2 Polyclonal Antibody (Product # PA5-60975).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of WAVE2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with WAVE2 Rabbit Polyclonal Antibody (Product # PA5-60975) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WAVE2 in human gallbladder using a WAVE2 Polyclonal Antibody (Product # PA5-60975) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WAVE2 in human esophagus using WAVE2 Polyclonal Antibody (Product # PA5-60975) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WAVE2 in human liver using WAVE2 Polyclonal Antibody (Product # PA5-60975) shows low expression as expected.