DM1207

antibody from Acris Antibodies GmbH

Targeting: CEACAM7 CEA, CGM2

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1207

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM1207, RRID:AB_977070

Product name

anti CEACAM7

Antibody type

Monoclonal

Antigen

CEACAM7/Fc fusion protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BAC2

Vial size

0.1 mg

Concentration

1.0 mg/ml

References

Submitted references

Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells.

Singer BB, Scheffrahn I, Kammerer R, Suttorp N, Ergun S, Slevogt H
PloS one 2010 Jan 18;5(1):e8747

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The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays.

Yue T, Goldstein IJ, Hollingsworth MA, Kaul K, Brand RE, Haab BB
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1697-707

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Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.

Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55

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Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.

Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergün S
Blood 2007 Dec 15;110(13):4223-33

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Interferon-gamma tempers the expression of carcinoembryonic antigen family molecules in human colon cells: a possible role in innate mucosal defence.

Fahlgren A, Baranov V, Frängsmyr L, Zoubir F, Hammarström ML, Hammarström S
Scandinavian journal of immunology 2003 Dec;58(6):628-41

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Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas.

Schölzel S, Zimmermann W, Schwarzkopf G, Grunert F, Rogaczewski B, Thompson J
The American journal of pathology 2000 Feb;156(2):595-605

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. FACS analysis of BOSC23 cells using BAC2 Cat.DM1207. BOSC23 cells were transiently transfected with an expression vectorencoding either CEACAM7 (Red curve) or an irrelevant protein (control transfectant). Binding of BAC2 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM7 transfected cells.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Members of the CEACAM family were expressed on BOSC cells after transient transfection withexpression vectors containing either the cDNA of CEACAM1, 5, 6, 7, 8 or a recombinant trans-membrane-anchored PSG1 fusion protein. Recognition of CEACAM3, CEACAM4 and PSG1 was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the con-structs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified BAC2 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM7 expressing cells (Red curves).