Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-47718 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOLR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FOLR2 is observed and less than 1% cross-reactivity with rhFOLR1 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human FOLR3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47718) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for FOLR3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of KG-1 human acute myelogenous leukemia cell line was stained with Sheep Anti-human FOLR3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47718) or isotype control antibodyopen histogram), followed by 637-conjugated Anti-Sheep IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.