PA5-45768

antibody from Invitrogen Antibodies

Targeting: FOLR2 FRβ

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-45768

Provider

Invitrogen Antibodies

Product name

FOLR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: LCEGLWSHSY KVSNYSRGSG RCIQMWFDSA QGNPNEEVAR FYAAAMHVNA Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 93%; Rat: 100%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Cancer Selective Target Degradation by Folate-Caged PROTACs.

Liu J, Chen H, Liu Y, Shen Y, Meng F, Kaniskan HÜ, Jin J, Wei W
Journal of the American Chemical Society 2021 May 19;143(19):7380-7387

---

Bortezomib-Loaded Mesoporous Silica Nanoparticles Selectively Alter Metabolism and Induce Death in Multiple Myeloma Cells.

Nigro A, Frattaruolo L, Fava M, De Napoli I, Greco M, Comandè A, De Santo M, Pellegrino M, Ricci E, Giordano F, Perrotta I, Leggio A, Pasqua L, Sisci D, Cappello AR, Morelli C
Cancers 2020 Sep 21;12(9)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of FOLR2 was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOLR2 Polyclonal Antibody (Product # PA5-45768) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 FOL-MSN-BTZ kills FR+ MM cells but not normal cells. ( A ) 50 mug of non-denatured proteins (for FR-alpha detection) and denatured proteins (for FR-beta detection) from total lysates of indicated cell lines were loaded and subjected to WB analysis. beta-actin was used as loading control. FOL-MSN-BTZ induces death in FR+ RPMI-8226 (RPMI) MM cells ( B ) but not in FR- BJhTERT normal cells ( C ). Cells were treated for 1h with FOL-MSN-BTZ or left untreated (C = control). MSN-FOL was used as negative control and the free drug BTZ as positive control. (*) p < 0.05 vs. control.