PA5-114387

antibody from Invitrogen Antibodies

Targeting: SEMA3B LUCA-1, SemA, sema5, SEMAA, semaV

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-114387

Provider

Invitrogen Antibodies

Product name

SEMA3B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human placenta tissue, human U2OS whole cell, human A549 whole cell, human U-87MG whole cell, rat thymus tissues, rat kidney tissue, mouse thymus tissue, mouse lung tissue, mouse small intestine tissue, mouse kidney tissue, mouse HEPA1-6 whole cel. IHC: human mammary cancer tissue, human colon cancer tissue, mouse brain tissue, mouse brain tissue, rat brain tissue, rat brain tissue. ICC/IF: A431 cell. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SEMA3B in A431 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SEMA3B in A431 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SEMA3B in A431 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEMA3B in paraffin-embedded rat brain tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEMA3B in paraffin-embedded rat brain tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEMA3B in paraffin-embedded mouse brain tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEMA3B in paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEMA3B in paraffin-embedded mouse brain tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEMA3B in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of SEMA3B in A549 cells. Samples were blocked with 10% normal goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of SEMA3B in A549 cells. Samples were blocked with 10% normal goat serum and incubated in SEMA3B polyclonal antibody (Product # PA5-114387) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).