Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [9]
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Validation data
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- Product number
- LS-C782222 - Provider product page
- Provider
- LSBio
- Product name
- INPPL1 / SHIP2 Antibody LS-C782222
- Antibody type
- Polyclonal
- Description
- Antigen Affinity purification
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) HeLa, 3) U-2 OS, 4) PC-3, 5) Caco-2, 6) A549 and 7) K562 lysate with SHIP2 antibody at 0.5ug/ml. Predicted molecular weight ~139 kDa (isoform 1), ~113 kDa (isoform 2).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot testing of rat 1) lung, 2) ovary, 3) brain and mouse 4) lung, 5) ovary and 6) brain lysate with SHIP2 antibody at 0.5ug/ml. Predicted molecular weight ~139 kDa (isoform 1), ~113 kDa (isoform 2).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE human ovarian cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC staining of FFPE human stomach cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human ovarian cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human stomach cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placenta with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse spleen with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat lung with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain with SHIP2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.