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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- A300-574A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INPPL1/SHIP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INPPL2/SHIP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INPPL2/SHIP2 antibody A300-574A (lot A300-574A-2) used for IP at 6 µg per reaction. INPPL2/SHIP2 was also immunoprecipitated by a previous lot of this antibody (A300-574A-1) and rabbit anti-INPPL2/SHIP2 antibody BL2376. For blotting immunoprecipitated INPPL2/SHIP2, A300-574A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse INPPL2/SHIP2 by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa and (50 µg) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody A300-574A (lot A300-574A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human expndtw-6 INPPL1/SHIP2 by immunohistochemistry. Sample: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 INPPL1/SHIP2 Cat. No. A300-574A Lot 1 (left) and Lot 2 (right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
