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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-19265 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRISP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, human and porcine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of HEK293 lysate overexpressing Human CRISP2 (c-term Myc tagged and loaded in Lane A&C) stained with PA5-19265 at a concentration of 1 µg/mL (lane A). Lane C was stained with anti-MYC Tag at a dilution of (1/1000). Lane B was loaded with mock-transfected HEK293 cells and stained using Product # PA5-19265 at a concentration of 1 mg/mL. Primary antibody incubation was performed for 1 hour and detection was performed by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CRISP2 in Human Testis using a CRISP2 monoclonal antibody (Product #PA5-19265) at 3.75 µg/mL. The Human Testis tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.
