Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-88885 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YTFLKVLNNM EIGASLFDEE GAKIVKDIMA KAQKNGVRIT FPVDFVTGDK FDENAQVGKA TVASGISPGW MGLDCGPESN KNHAQVVAQA RLIVWNGPLG VFEWDAFAKG TKALMDEIV; Positive Samples: U-87MG, Jurkat, HeLa, HepG2, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain; Cellular Location: Cytoplasm
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using PGK2 Polyclonal antibody (Product # PA5-88885) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PGK2 in extracts of various cell lines using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PGK2 in extracts of various cell lines using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PGK2 was performed in C6 cells using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PGK2 was performed in L929 cells using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGK2 in paraffin-embedded human mammary cancer using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGK2 in paraffin-embedded rat testis using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGK2 in paraffin-embedded mouse testis using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100.