Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ANR-185-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tetraspanin-3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:400). 3-4. Anti- Tetraspanin-3 (extracellular) Antibody , preincubated with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6): - 1-3. Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:200). 4-6. Anti- Tetraspanin-3 (extracellular) Antibody , preincubated with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Tetraspanin-3 in rat cortex. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:300), followed by donkey Anti-rabbit-biotin and streptavidin-Cy3. A. Tetraspanin-3 immunoreactivity (red) appears in neuron profiles (arrows). B. Pre-incubation of the Antibody with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Tetraspanin-3 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (2.5μg) + goat- Anti-rabbit-FITC.
