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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSPAN3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, mouse kidney.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TSPAN3 Polyclonal Antibody (Product # PA5-28514). Sample (50 µg of whole cell lysate). Lane A: mouse kidney. 12% SDS PAGE. TSPAN3 Polyclonal Antibody (Product # PA5-28514) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TSPAN3 Polyclonal Antibody (Product # PA5-28514). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. 12% SDS PAGE. TSPAN3 Polyclonal Antibody (Product # PA5-28514) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TSPAN3 Polyclonal Antibody detects TSPAN3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TSPAN3 protein stained by TSPAN3 Polyclonal Antibody (Product # PA5-28514) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TSPAN3 Polyclonal Antibody detects TSPAN3 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. TSPAN3 Polyclonal Antibody (Product # PA5-28514) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
