MA5-25907

antibody from Invitrogen Antibodies

Targeting: SPG7 CAR, CMAR, SPG5C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-25907

Provider

Invitrogen Antibodies

Product name

SPG7 Monoclonal Antibody (OTI1C12)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1C12

Vial size

100 µL

Concentration

0.86 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPG7 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with SPG7 (Product # MA5-25907) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPG7 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with SPG7 (Product # MA5-25907) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SPG7 in COS7 cells. Cells were transfected with a plasmid overexpressing SPG7 and probed with a SPG7 monoclonal antibody (Product # MA5-25907).