MAB13351

antibody from Novus Biologicals

Targeting: IL17F IL-17F, ML-1, ML1

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB13351

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-17F Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-17F in direct ELISAs. Does not cross-react with recombinant human IL-17A, B, C, D, or E.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.

Chan YR, Chen K, Duncan SR, Lathrop KL, Latoche JD, Logar AJ, Pociask DA, Wahlberg BJ, Ray P, Ray A, Pilewski JM, Kolls JK
The Journal of allergy and clinical immunology 2013 Apr;131(4):1117-29, 1129.e1-5

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TH17 cytokines induce human airway smooth muscle cell migration.

Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
The Journal of allergy and clinical immunology 2011 Apr;127(4):1046-53.e1-2

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Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.

Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 1;182(5):3112-20

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17F in Activated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours (dark orange filled histogram), then stained with Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13351) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.