Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2057 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17F Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-17F in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human IL-17F, recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_665855- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mucin 1 protects against severe Streptococcus pneumoniae infection.
T-lymphocytes enable osteoblast maturation via IL-17F during the early phase of fracture repair.
Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.
Dhar P, Ng GZ, Dunne EM, Sutton P
Virulence 2017 Nov 17;8(8):1631-1642
Virulence 2017 Nov 17;8(8):1631-1642
T-lymphocytes enable osteoblast maturation via IL-17F during the early phase of fracture repair.
Nam D, Mau E, Wang Y, Wright D, Silkstone D, Whetstone H, Whyne C, Alman B
PloS one 2012;7(6):e40044
PloS one 2012;7(6):e40044
Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.
Ishigame H, Kakuta S, Nagai T, Kadoki M, Nambu A, Komiyama Y, Fujikado N, Tanahashi Y, Akitsu A, Kotaki H, Sudo K, Nakae S, Sasakawa C, Iwakura Y
Immunity 2009 Jan 16;30(1):108-19
Immunity 2009 Jan 16;30(1):108-19
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-17F in Mouse Th17 Cells. IL-17F was detected in immersion fixed mouse T helper 17 (Th17) cells stimulated with PMA and calcium ionomycin using Goat Anti-Mouse IL-17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2057) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
