16-7169-82

antibody from Invitrogen Antibodies
Targeting: IL17F IL-17F, ML-1, ML1
Provider product page for 16-7169-82
Blocking/Neutralizing
Functional assay
Other assay

Antibody data

Product number
16-7169-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
IL-17F Monoclonal Antibody (SHLR17), Functional Grade, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This SHLR17 monoclonal antibody reacts with human interleukin (IL)-17F. IL-17F is a homodimeric, 34 kDa member of the IL-17 family, which includes IL-17(A), B, C, D, E (IL-25). Within this family, IL-17A and IL-17F are produced preferentially by T helper 17 (Th17) cells, with other members produced more widely. IL-17F and IL-17A have many overlapping functions, but knockout studies have shown each to have independent functions as well. In vitro cultured Th17 cells have been shown to express IL-17F and IL-17A homodimers and IL-17A/F heterodimers depending on the culture conditions and their differentiation state. A heterodimer of IL-17RA and IL-17RC has been shown to bind IL-17F, resulting in target cell-secretion of pro-inflammatory cytokines and chemokines, as well as neutrophil recruitment. This SHLR17 monoclonal antibody neutralizes the bioactivity of IL-17F homodimers without affecting IL-17A/F heterodimers. Applications Reported: This SHLR17 monoclonal antibody inhibits the activity of human IL-17F. Applications Tested: The ND50 of SHLR17, as measured by the inhibition of mouse IL-6 induction in NIH/3T3 cells, is 0.5-4 µg/mL in the presence of 1 µg/mL of Human IL-17F Recombinant Protein. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. This antibody should be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity
Human
Host
Rat
Isotype
IgG
Antibody clone number
SHLR17
Vial size
100 µg
Concentration
1 mg/mL
Storage
4°C
IL17F protein structure - 16-7169-82 shown in red.