25-7169-42

antibody from Invitrogen Antibodies

Targeting: IL17F IL-17F, ML-1, ML1

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

25-7169-42

Provider

Invitrogen Antibodies

Product name

IL-17F Monoclonal Antibody (SHLR17), PE-Cyanine7, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The SHLR17 monoclonal antibody reacts with human interleukin (IL)-17F. IL-17F is a homodimeric, 34 kDa member of the IL-17 family, which includes IL-17(A), B, C, D, E (IL-25). Within this family, IL-17A and IL-17F are produced preferentially by T helper 17 (Th17) cells, with other members produced more widely. IL-17F and IL-17A have many overlapping functions, but knockout studies have shown each to have independent functions as well. \i{In vitro\i} cultured Th17 cells have been shown to express IL-17F and IL-17A homodimers and IL-17A/F heterodimers depending on the culture conditions and their differentiation state. A heterodimer of IL-17RA and IL-17RC has been shown to bind IL-17F, resulting in target cell-secretion of pro-inflammatory cytokines and chemokines, as well as neutrophil recruitment. Applications Reported: This SHLR17 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SHLR17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th17 polarized human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

SHLR17

Vial size

100 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Interplay between SMAD2 and STAT5A is a critical determinant of IL-17A/IL-17F differential expression.

Corral-Jara KF, Chauvin C, Abou-Jaoudé W, Grandclaudon M, Naldi A, Soumelis V, Thieffry D
Molecular biomedicine 2021 Apr 1;2(1):9

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A Quantitative Multivariate Model of Human Dendritic Cell-T Helper Cell Communication.

Grandclaudon M, Perrot-Dockès M, Trichot C, Karpf L, Abouzid O, Chauvin C, Sirven P, Abou-Jaoudé W, Berger F, Hupé P, Thieffry D, Sansonnet L, Chiquet J, Lévy-Leduc C, Soumelis V
Cell 2019 Oct 3;179(2):432-447.e21

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Human Th17 cells comprise heterogeneous subsets including IFN-gamma-producing cells with distinct properties from the Th1 lineage.

Boniface K, Blumenschein WM, Brovont-Porth K, McGeachy MJ, Basham B, Desai B, Pierce R, McClanahan TK, Sadekova S, de Waal Malefyt R
Journal of immunology (Baltimore, Md. : 1950) 2010 Jul 1;185(1):679-87

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracellular staining of CD4-enriched Th17-polarized (using Human IL-23 Recombinant Protein (Product # 14-8239-63), normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human IL-17F PE-Cyanine7. Cultures were treated with Protein Transport Inhibitor Cocktail alone (Product # 00-4980-03) (left) or Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours prior to intracellular staining using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 Experimental validation of CD4 + T cell phenotypes. Human naive T cells were differentiated for 5 days in the presence of polyclonal activation (anti-CD3/anti-CD28 beads). Cells were cultured in the presence of different cytokine inputs: proTh1 (IL-12), IL-1beta, IL-23, IL-12 + IL-1beta, IL-1beta + IL-23, IL-12 + IL-1beta + IL-23, proTh17 (IL-1beta + IL-6 + IL-23 + TGF-beta). Cells exposed only to polyclonal stimulation were considered as Th0. Differentiated cells were then subjected to flow cytometry analysis. a Dot plots representing the IL-17A and IL-17F cells in live CD4 + cells are shown on the left. Dot plots representing the IL-17F and IFN-gamma cells in live CD4 + cells are shown on the right. Representative data from three independent experiments are shown. Numbers denotes frequency of gated cells. b The frequency of cells for each subset in Apannel a is shown. Graphs represent mean +- SD, N = 12, *, ** and *** denotes p < 0.05, p < 0.01 and p < 0.001, respectively (Wilcoxon test). c Cells were submitted to a second round of polarization in the presence of the cytokine inputs: IL-1beta, IL-12, IL-23, IL-12 + IL-1beta, proTh17, for two additional days. Dot plots representing the IL-17A and IL-17F cells in live CD4 + cells and frequency of cells are shown. d The frequency of cells for each subset in Cpannel c is shown. Graphs represent mean +- SD, N = 6, * represents p < 0.05 (Wilcoxon test)