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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 516603 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#516603, RRID:AB_10730721
- Product name
- Alexa Fluor® 488 anti-human IL-17F [Poly5166]
- Antibody type
- Polyclonal
- Antigen
- IL-17F
- Description
- IL-17F is part of the IL-17 cytokine family which consists of at least seven structurally related proteins (IL-17A, B, C, D, E, F, and A/F). IL-17F homodimer adopts a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for inter-chain bonding. IL-17F is most closely related to IL-17A, sharing 50% amino acid sequence homology. It is expressed by Th17 cells, along with IL-17A homodimer and IL-17A/F heterodimer. IL-17F has been shown to inhibit endothelial cell angiogenesis and induce increased production of IL-2, TGF-β, and MCP-1, playing a critical role in the regulation of inflammatory reactions.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Green dye
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- PMA + ionomycin-stimulated (6 hours in the presence of monensin) human peripheral blood lymphocytes surface stained with CD3 APC, fixed and permeabilized and stained with IL-17F (clone Poly5166) Alexa Fluor® 488 (top) or blocked by pre-incubation purified IL-17F (Poly5166) (bottom).
- Conjugate
- Green dye
