MA5-16229

antibody from Invitrogen Antibodies

Targeting: IL17F IL-17F, ML-1, ML1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16229

Provider

Invitrogen Antibodies

Product name

IL-17F Monoclonal Antibody (4H1629)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4H1629

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Exaggerated IL-17A activity in human in vivo recall responses discriminates active tuberculosis from latent infection and cured disease.

Pollara G, Turner CT, Rosenheim J, Chandran A, Bell LCK, Khan A, Patel A, Peralta LF, Folino A, Akarca A, Venturini C, Baker T, Ecker S, Ricciardolo FLM, Marafioti T, Ugarte-Gil C, Moore DAJ, Chain BM, Tomlinson GS, Noursadeghi M
Science translational medicine 2021 May 5;13(592)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Active TB is characterized by elevated Th17 cells at the site of TST. (A) IL-17F immunohistochemistry (brown) in TST of patients with active TB. Black arrowheads point to mononuclear cells that express IL-17F and white arrowheads point to polymorphonuclear cells that do not express IL-17F. (B) Th17 and Th1 cell module enrichment in TST samples relative to saline injection in patients with active or latent TB. Analyses were performed on samples from 48 and 191 participants with active and latent TB, respectively. Horizontal lines and error bars on scatter dot plots represent medians with 95% confidence interval. (C and D) Expression of T cells, Th17 cells, and IL-17A bioactivity modules from the site of human TB granulomata (n=5) relative to healthy lung tissue (n=2) (dataset GSE20050) (C) and in human Mtb-infected lymph nodes (LN) (n=9) relative to reactive lymph nodes that do not display evidence of granulomatous inflammation or cancer (n=10) (D) (dataset E-MTAB-2547). Violin plots represent frequency distribution of all samples, with bold lines representing median values. All p values were calculated by Mann-Whitney tests.