MA5-16229

antibody from Invitrogen Antibodies

Targeting: IL17F IL-17F, ML-1, ML1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16229

Provider

Invitrogen Antibodies

Product name

IL-17F Monoclonal Antibody (4H1629.1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

The human IL17F protein shows 60% sequence similarity with Rat and 57% with Mouse. Does not react with Rat or Mouse recombinant IL17F proteins. Immunogen sequence: MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4H1629.1

Vial size

100 μg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Exaggerated IL-17A activity in human in vivo recall responses discriminates active tuberculosis from latent infection and cured disease.

Pollara G, Turner CT, Rosenheim J, Chandran A, Bell LCK, Khan A, Patel A, Peralta LF, Folino A, Akarca A, Venturini C, Baker T, Ecker S, Ricciardolo FLM, Marafioti T, Ugarte-Gil C, Moore DAJ, Chain BM, Tomlinson GS, Noursadeghi M
Science translational medicine 2021 May 5;13(592)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IL-17F in formalin-fixed paraffin-embedded human colon tissue. Samples were incubated in IL-17F monoclonal antibody (Product # MA5-16229) using a dilution of 5 µg/mL. Isotype control (top left) and IL-17F antibody (bottom left and right).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IL-17F in a tissue section of human lymph node cancer. Samples were incubated in IL-17F monoclonal antibody (Product # MA5-16229) using a dilution of 5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IL-17F in a tissue section of human stomach cancer. Samples were incubated in IL-17F monoclonal antibody (Product # MA5-16229) using a dilution of 5 µg/mL. Diffused cytoplasmic staining was observed in the tissue section and interestingly, the expression levels in cancerous tissue were found to be weaker than the parallel stained normal tissue section.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IL-17F in a tissue section of normal human stomach. Samples were incubated in IL-17F monoclonal antibody (Product # MA5-16229) using a dilution of 5 µg/mL. Cytoplasmic expression was observed all over the tissue section and the staining was found to be more intense expecially in the chief cells of the glands.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Active TB is characterized by elevated Th17 cells at the site of TST. (A) IL-17F immunohistochemistry (brown) in TST of patients with active TB. Black arrowheads point to mononuclear cells that express IL-17F and white arrowheads point to polymorphonuclear cells that do not express IL-17F. (B) Th17 and Th1 cell module enrichment in TST samples relative to saline injection in patients with active or latent TB. Analyses were performed on samples from 48 and 191 participants with active and latent TB, respectively. Horizontal lines and error bars on scatter dot plots represent medians with 95% confidence interval. (C and D) Expression of T cells, Th17 cells, and IL-17A bioactivity modules from the site of human TB granulomata (n=5) relative to healthy lung tissue (n=2) (dataset GSE20050) (C) and in human Mtb-infected lymph nodes (LN) (n=9) relative to reactive lymph nodes that do not display evidence of granulomatous inflammation or cancer (n=10) (D) (dataset E-MTAB-2547). Violin plots represent frequency distribution of all samples, with bold lines representing median values. All p values were calculated by Mann-Whitney tests.