AF1335

antibody from R&D Systems

Targeting: IL17F IL-17F, ML-1, ML1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1335

Provider

R&D Systems

Product name

Human/Primate IL-17F Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human IL-17F in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, and recombinant mouse IL-17F is observed.

Reactivity

Human, Simian

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAK83350


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.

Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas AK, Chouiali F, Nair P, Baglole CJ, Hamid Q, Eidelman DH
Respiratory research 2014 Nov 27;15:145

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Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.

Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
Chest 2010 Nov;138(5):1140-7

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Tripeptidyl-peptidase II controls DNA damage responses and in vivo gamma-irradiation resistance of tumors.

Hong X, Lei L, Künert B, Naredla R, Applequist SE, Grandien A, Glas R
Cancer research 2007 Aug 1;67(15):7165-74

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-17F in Human Tonsil. IL-17F was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human/Primate IL-17F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1335) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.