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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-85571 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (90%), Rat (91%), Chicken (80%), Rhesus Monkey (98%), Bovine (97%). Positive Control: Jurkat, Raji, NCI-H929. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C1D in A) Jurkat whole cell lysate, B) Raji whole cell lysate, C) NCI-H929 whole cell lysate using C1D polyclonal antibody (Product # PA5-85571) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C1D Polyclonal Antibody detects C1D protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.15 % SDS-PAGE. C1D Polyclonal Antibody (Product # PA5-85571) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C1D Polyclonal Antibody detects C1D protein at nucleus, nucleolus and cytoplasm by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C1D protein stained by C1D Polyclonal Antibody (Product # PA5-85571) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C1D Polyclonal Antibody detects C1D protein at nucleolus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. C1D Polyclonal Antibody (Product # PA5-85571) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C1D Polyclonal Antibody detects C1D protein at nucleolus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. C1D Polyclonal Antibody (Product # PA5-85571) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
