Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP52110PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52110PU-N, RRID:AB_11148612
- Product name
- anti 17-beta-HSD12 / HSD17B12 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 133~163 amino acids from the Center region of human 17-beta-HSD12 / HSD17B12
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of 17-beta-HSD12 / HSD17B12Â Antibody (Center) Cat.-No AP52110PU-NÂ in A2058 cell line lysates (35ug/lane). HSD17B12 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with 17-beta-HSD12 / HSD17B12 Antibody (Center) Cat.-No AP52110PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A2058 cells using 17-beta-HSD12 / HSD17B12 Antibody (Center) Cat.-No AP52110PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.