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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- SM6017S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM6017S, RRID:AB_1621865
- Product name
- anti RASSF1 (Isoform A)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human RASSF1A (1-340 aa), purified from E. coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F3
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The extracts of HEK293 (1), A549 (2) and HeLa (3) cells were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human RASSF1A antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of HEk293(1), A549(2) and HeLa(3) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunocytochemistry: A431 cells were labeled with anti-RASSF1a antibody (clone 3F3) and detection was using a biotinylated secondary antibody and Texas-red conjugated Streptavidin.
