H00114327-M09

antibody from Abnova Corporation

Targeting: EFHC1 EJM, EJM1, FLJ10466, POC9, RIB72

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00114327-M09

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00114327-M09, RRID:AB_1674220

Product name

EFHC1 monoclonal antibody (M09), clone 4E7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant EFHC1.

Antigen sequence

DDTVEIREVHERNDGRDPFPLLMNRQRVPKVLVEN
AKNFPQCVLEISDQEVLEWYTAKDFIVGKSLTILG
RTFFIYDCDPFTRRYYKEKFGITDLPRIDVSKREP
PPVK


Isotype

IgG

Antibody clone number

4E7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EFHC1 expression in transfected 293T cell line by EFHC1 monoclonal antibody (M09), clone 4E7.Lane 1: EFHC1 transfected lysate (Predicted MW: 73.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EFHC1 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol