A301-980A

antibody from Invitrogen Antibodies

Targeting: FANCA FA-H, FAA, FACA, FAH, FANCH

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-980A

Provider

Invitrogen Antibodies

Product name

FANCA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FANCA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, A-549, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCA antibody (Product # A301-980A; lot A301-980A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FANCA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FANCA antibody A301-980A (lot A301-980A-2) used for IP at 6 µg per reaction. FANCA was also immunoprecipitated by a previous lot of this antibody (lot A301-980A-1). For blotting immunoprecipitated FANCA, A301-980A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FANCA by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCA antibody A301-980A (lot A301-980A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FANCA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FANCA antibody (Product # A301-980A; lot A301-980A-3) used for IP at 6 µg per reaction. FANCA was also immunoprecipitated by a previous lot of this antibody (lot A301-980A-2). For blotting immunoprecipitated FANCA, Product # A301-980A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.