PA5-21670

antibody from Invitrogen Antibodies

Targeting: GSTA2 GST2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21670

Provider

Invitrogen Antibodies

Product name

GSTA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2. Predicted reactivity: Pig (82%), Rhesus Monkey (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Glutathione S-transferase A2 promotes hepatocellular carcinoma recurrence after liver transplantation through modulating reactive oxygen species metabolism.

Ng KT, Yeung OW, Lam YF, Liu J, Liu H, Pang L, Yang XX, Zhu J, Zhang W, Lau MYH, Qiu WQ, Shiu HC, Lai MK, Lo CM, Man K
Cell death discovery 2021 Jul 21;7(1):188

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GSTA2 Polyclonal Antibody (Product # PA5-21670). Sample (30 µg of whole cell lysate). A: Hep G2. 12% SDS PAGE. GSTA2 Polyclonal Antibody (Product # PA5-21670) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of glutathione S-transferase alpha 2 in methanol-fixed Hep G2 cells using a glutathione S-transferase alpha 2 polyclonal antibody (Product # PA5-21670) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed Hep G2, using GSTA1/GSTA2/GSTA3 antibody (Product # PA5-21670) at 1:500 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 The expression significance of GSTA2 in HCC. A Expression of GSTA2 mRNA in the tumor (T) and non-tumor (NT) liver tissues of HCC patients, and in the liver tissues of healthy donors. B Western blot analysis of GSTA2 protein in paired tumor (T) and non-tumor (NT) tissues of HCC patients. C The correlation analysis of the expression level of tumoral GSTAs mRNA and tumor size, the presence of venous infiltration, and pTNM stage of HCC patients. D The expression level of tumoral GSTA2 mRNAs between less aggressive and more aggressive tumors after liver transplantation in rats. E The expression of GSTA2 mRNA and protein among different HCC cell lines.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 Overexpression of GSTA2 promoted HCC growth and invasion. A The expression level of GSTA2 mRNA and protein in GSTA2-overexpressing HCC cells. B MTT assay of HCC cells after endogenously overexpressing GSTA2 by lentivirus (Lent-GSTA2) or administrating human recombinant GSTA2 protein (rGSTA2). C Colony-forming assay. D Matrigel invasion assay. Scale bar: 200 muM. E Wound healing migration assay. Scale bar: 1000 muM. F Western blot analysis of EMT-associated proteins.. Control, Hep3B-control cells; Lenti-GSTA2, Hep3B-LentiGSTA2 cells; rGSTA2, human recombinant GSTA2 protein. * P < 0.05; ** P < 0.01.