MA5-27682

antibody from Invitrogen Antibodies

Targeting: KCNC4 C1orf30, HKSHIIIC, Kv3.4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27682

Provider

Invitrogen Antibodies

Product name

KV3.4 (KCNC4) Monoclonal Antibody (S72-16)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S72-16

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KV3.4 (KCNC4) in rat brain membrane lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with KV3.4 (KCNC4) monoclonal antibody (Product # MA5-27682) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KV3.4 (KCNC4) in human HaCaT cells. Sample was fixed with 100% cold methanol (10 minutes at -20°C), incubated with KV3.4 (KCNC4) monoclonal antibody (Product # MA5-27682) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) secondary antibody at a dilution of 1:50 (1 hour at RT).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KV3.4 (KCNC4) in paraffin-embedded mouse back skin. Sample was fixed with Bouin's fixative solution, incubated with KV3.4 (KCNC4) monoclonal antibody (Product # MA5-27682) using a dilution of 1:100 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.