PA5-118677

antibody from Invitrogen Antibodies

Targeting: APLF C2orf13, MGC47799, Xip1, ZCCHH1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118677

Provider

Invitrogen Antibodies

Product name

Phospho-APLF (Ser116) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-APLF (Ser116) in starvation treated K562 cells using a Phospho-APLF (Ser116) polyclonal antibody (Product # PA5-118677). The -/+ symbols identify the absence or presence of N peptide (non-phospho peptide) and P peptide(phospho peptide).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-APLF (Ser116) in Human breast tissue using a Phospho-APLF (Ser116) polyclonal antibody (Product # PA5-118677). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-APLF (Ser116) in Mouse liver tissue using a Phospho-APLF (Ser116) polyclonal antibody (Product # PA5-118677). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-APLF (Ser116) in Rat brain tissue using a Phospho-APLF (Ser116) polyclonal antibody (Product # PA5-118677). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.