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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-30044 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CACNA2D2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.35 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CACNA2D2 Antibody [NBP2-30044] - Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the CACNA2D2 antibody detected the CACNA2D2 protein (arrow).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CACNA2D2 Antibody [NBP2-30044] - Anti-CACNA2D2 Antibody (Center) at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CACNA2D2 Antibody [NBP2-30044] - Fluorescent image of U251 cell stained with CACNA2D2 Antibody (Center)(NBP2-30044).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25, 1 h at 37). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37).CACNA2D2 immunoreactivity is localized to Cytoplasm significantly.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CACNA2D2 Antibody [NBP2-30044] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
