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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF1385 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RELT/TNFRSF19L Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human RELT/TNFRSF19L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhTAJ, rhCD30, rhDR3, rhDR6, rhTNF RI, rhTNF RII, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, and recombinant mouse OX40 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q969Z4- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RELT induces cellular death in HEK 293 epithelial cells.
Cusick JK, Mustian A, Goldberg K, Reyland ME
Cellular immunology 2010;261(1):1-8
Cellular immunology 2010;261(1):1-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human RELT/TNFRSF19L by Western Blot. Western blot shows lysates of human bone marrow tissue and human lymph node tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RELT/TNFRSF19L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1385) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RELT/TNFRSF19L at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
