PA5-47913

antibody from Invitrogen Antibodies

Targeting: GKN1 AMP18, BRICD1, CA11

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47913

Provider

Invitrogen Antibodies

Product name

GKN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 10% cross-reactivity with recombinant human Gastrokine 1 is observed. Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

Reactivity

Human, Mouse

Host

Sheep

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells' heterogeneity.

Schlesinger Y, Yosefov-Levi O, Kolodkin-Gal D, Granit RZ, Peters L, Kalifa R, Xia L, Nasereddin A, Shiff I, Amran O, Nevo Y, Elgavish S, Atlan K, Zamir G, Parnas O
Nature communications 2020 Sep 9;11(1):4516

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 Potential metaplastic cell-cell interactions and co-localization of different metaplastic cell types to the same PanINs. a - c Immunostaining of histological sections with anti-Dclk1 (red) and anti-Muc5ac (brown) antibodies. Arrows mark representative cells in the PanINs that stained positive with anti-Dclk1 (green arrow) or with anti-Muc5ac (black arrow). a Section taken from control, 5 months PT mouse, b section taken from 5 months PRT mouse, c section taken from 9 months PRT mouse. Scale bars 100 um. d Violin plot showing the expression level (normalized expression) of a selected set of secreted molecules and receptors. On the x -axis: Early acinar/ductal cells (control, 17 days and 6 weeks PTI samples), late (all other time points), and clusters of metaplastic cells. e Dot plot showing the expression of a set of receptors ( x -axis) across the different subset of immune cells ( y -axis). Immune cells subtypes are determined based on data presented in Fig. 7 . The average expression level is shown by the size of the dot and the percentage of cells is indicated by the color. f , i Immunostaining of histological sections of the pancreas, double stain with anti-Tgfb1 (red) and with anti-Gkn1 (brown), representative cell marked with arrows, anti-Tgfb1 (green arrow), and anti-Gkn1 (black arrow), f control, 5 months PT mouse, i 5 months PRT mouse. g 5 months PRT mouse section, stained with anti-Tgfb1, h 5 months PRT mouse section, stained with anti-Gkn1. i . Scale bars 10