AP09310PU-N

antibody from Acris Antibodies GmbH

Targeting: FBXO9 FBX9, NY-REN-57

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09310PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09310PU-N, RRID:AB_2035469

Product name

anti FBXO9 (431-447)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 431-447 of human FBOX9 protein

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Xenopus, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.53 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using affinity purified anti-FBOX9 antibody shows detection of a band at ~100 kDa (arrowhead) believed to correspond to FBOX9 present in a MCF7 whole cell lysate (lane 1). Specific band reactivity is greatly diminished when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red). IRDye (TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.