A305-396A

antibody from Invitrogen Antibodies

Targeting: AP1S1 AP19, CLAPS1, EKV3, SIGMA1A, WUGSC:H_DJ0747G18.2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A305-396A

Provider

Invitrogen Antibodies

Product name

AP1S1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human AP1S1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AP1S1 antibody A305-396A (lot A305-396A-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated AP1S1, A305-396A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse AP1S1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AP1S1 antibody A305-396A (lot A305-396A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.