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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- ABIN2616363 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ankyrin Repeat Domain 2 (Stretch Responsive Muscle) (ANKRD2) antibody
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- YAS11
- Vial size
- 100 μg
- Storage
- Store at -20°C.
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Histone H3 acetyl Lys14 pAb tested by Western blot. HeLa acid extract (10 μg per lane) was probed with Histone H3 acetyl Lys14 pAb (2 μg/ml dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.
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- antibodies-online (provider)
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- Experimental details
- Histone H3 acetyl Lys14 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys14 pAb for acetyl Lys14 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 μg/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys9 peptide. Lane 2: unmodified Lys9 peptide. Lane 3: acetyl-Lys14 peptide. Lane 4: unmodified Lys14 peptide. Lane 5: acetyl-Lys18 peptide. Lane 6: acetyl-Lys23 peptide. Lane 7: acetyl-Lys27 peptide. No detection of peptides acetylated at either lysine 4, 36, 37, 64 or 79 of histone H3 was observed (data not shown).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 acetyl Lys14 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 5 μg of Histone H3 acetyl Lys14 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
