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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- MA5-47011 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- YTHDF2 Recombinant Rabbit Monoclonal Antibody (HL1355)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Store as concentrated solution. Centrifuge briefly prior to opening vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1355
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of YTHDF2 in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: YTHDF2 stained by YTHDF2 monoclonal antibody (Product # MA5-47011) diluted at 1:500. Blue: Fluoroshield with DAPI Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of YTHDF2 in paraffin-embedded mouse intestine. Samples were incubated in YTHDF2 Monoclonal antibody (Product # MA5-47011). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of YTHDF2 in paraffin-embedded rat colon. Samples were incubated in YTHDF2 Monoclonal antibody (Product # MA5-47011). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of YTHDF2 in paraffin-embedded mouse hippocampus. Samples were incubated in YTHDF2 Monoclonal antibody (Product # MA5-47011). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of YTHDF2 from Raji whole cell extracts. Western blot analysis was performed using 5 µg YTHDF2 monoclonal antibody (Product # MA5-47011) followed by EasyBlot HRP-conjugated anti-rabbit IgG antibody.
