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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- AP1321a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1321a, RRID:AB_2228189
- Product name
- BNIP3 Antibody (BH3 Domain Specific)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Autophagy and Bcl-2/BNIP3 death regulatory pathway in non-small cell lung carcinomas.
Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress.
Karpathiou G, Sivridis E, Koukourakis M, Mikroulis D, Bouros D, Froudarakis M, Bougioukas G, Maltezos E, Giatromanolaki A
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2013 Jul;121(7):592-604
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2013 Jul;121(7):592-604
Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress.
Metukuri MR, Beer-Stolz D, Namas RA, Dhupar R, Torres A, Loughran PA, Jefferson BS, Tsung A, Billiar TR, Vodovotz Y, Zamora R
American journal of physiology. Gastrointestinal and liver physiology 2009 Mar;296(3):G499-509
American journal of physiology. Gastrointestinal and liver physiology 2009 Mar;296(3):G499-509
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- The anti-NIP3 BH3 domain Pab (Cat. #AP1321a) is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1321a BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells."
- Primary Ab dilution
- 1:500
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody Cat # AP1321a. Data courtesy of Ruben Zamora, University of Pittsburgh."
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
