Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB4147 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal BNIP3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BNIP3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human BNIP3L is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references High content screening identifies monensin as an EMT-selective cytotoxic compound.
Autophagy Induction Results in Enhanced Anoikis Resistance in Models of Peritoneal Disease.
Vanneste M, Huang Q, Li M, Moose D, Zhao L, Stamnes MA, Schultz M, Wu M, Henry MD
Scientific reports 2019 Feb 4;9(1):1200
Scientific reports 2019 Feb 4;9(1):1200
Autophagy Induction Results in Enhanced Anoikis Resistance in Models of Peritoneal Disease.
Chen JL, David J, Cook-Spaeth D, Casey S, Cohen D, Selvendiran K, Bekaii-Saab T, Hays JL
Molecular cancer research : MCR 2017 Jan;15(1):26-34
Molecular cancer research : MCR 2017 Jan;15(1):26-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human BNIP3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl2 for 18 hours. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human BNIP3 Monoclonal Antibody (Catalog # MAB4147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BNIP3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.