F42817

antibody from NSJ Bioreagents

Targeting: BNIP3 Nip3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F42817

Provider

NSJ Bioreagents

Product name

BNIP3 Antibody

Antibody type

Polyclonal

Description

This highly specific BNIP3 antibody is suitable for use in Immunofluorescence/Western blot/Immunohistochemistry/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the BNIP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

The BNIP3 antibody western blot analysis of Ramos cell lysate (lane 1) and in mouse brain tissue lysate (2).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent confocal image of HepG2 cells stained with BNIP3 antibody at 1:50. BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Freshly isolated mouse hepatocytes were cultured under normoxic conditions for 6 hr. and stained with BNIP3 antibody; Red: F-actin, Blue: ATP-synthase, Green: BNIP3. (Courtesy of Ruben Zamora, University of Pittsburgh.)

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human breast carcinoma tissue stained with the BNIP3 antibody