Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- F42817 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- BNIP3 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 152-187 from the human protein (BH3 domain) was used as the immunogen for this BNIP3 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the BNIP3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- The BNIP3 antibody western blot analysis of Ramos cell lysate (lane 1) and in mouse brain tissue lysate (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HepG2 cells stained with BNIP3 antibody at 1:50. BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Freshly isolated mouse hepatocytes were cultured under normoxic conditions for 6 hr. and stained with BNIP3 antibody; Red: F-actin, Blue: ATP-synthase, Green: BNIP3. (Courtesy of Ruben Zamora, University of Pittsburgh.)
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma tissue stained with the BNIP3 antibody