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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-56150 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56150, RRID:AB_2274960
- Product name
- Rabbit Polyclonal BNIP3 Antibody
- Antibody type
- Polyclonal
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.
Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BNIP3 Antibody - Unpurified [NB100-56150] - BNIP3 Antibody [NB100-56150] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using BNIP3 antibody at 1:1000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Mitochondrial staining was observed. Staining was performed by Histowiz.
