PA5-11402

antibody from Invitrogen Antibodies

Targeting: BNIP3 Nip3

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-11402

Provider

Invitrogen Antibodies

Product name

BNIP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

1.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Blockade of the CD93 pathway normalizes tumor vasculature to facilitate drug delivery and immunotherapy.

Sun Y, Chen W, Torphy RJ, Yao S, Zhu G, Lin R, Lugano R, Miller EN, Fujiwara Y, Bian L, Zheng L, Anand S, Gao F, Zhang W, Ferrara SE, Goodspeed AE, Dimberg A, Wang XJ, Edil BH, Barnett CC, Schulick RD, Chen L, Zhu Y
Science translational medicine 2021 Jul 28;13(604)

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Proximity-dependent initiation of hybridization chain reaction.

Koos B, Cane G, Grannas K, Löf L, Arngården L, Heldin J, Clausson CM, Klaesson A, Hirvonen MK, de Oliveira FM, Talibov VO, Pham NT, Auer M, Danielson UH, Haybaeck J, Kamali-Moghaddam M, Söderberg O
Nature communications 2015 Jun 12;6:7294

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using a BNIP3 polyclonal antibody (Product # PA5-11402). HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with a BNIP3 polyclonal antibody (Product # PA5-11402) (1:500, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mouse hepatocytes using a BNIP3 polyclonal antibody (Product # PA5-11402) at a dilution of 1:50. Freshly isolated mouse hepatocytes plated on coverslips (2x10^5 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 In situ prox-HCR. ( a-d ) Technical controls for the E-cadherin/beta-catenin interaction. Strong membranous signal could be observed in HT29 cells when both primary antibodies were applied ( a ), while omitting either one of the primary antibodies ( b , c ) or both ( d ) did not yield visible signal. Phosphorylation of platelet-derived growth factor receptor-beta (PDGFR-beta) could be shown in BjHTert cells following stimulation with 100 ng ml -1 ( f ), while expression of phosphorylated receptor was low in non-stimulated cells ( e ). ProxHCR was used to visualize the induction of autophagy following starvation and incubation with CoCl 2 in Caco cells ( g-j ). Although untreated cells showed only low basal activity ( g ) of BCL2-BNIP3 interaction, a highly increased signal could be observed in treated cells ( h ). The same holds true for LC3-SQSTM1 interaction ( i , j ). The MEK-ERK interaction could be induced in A431 cells by stimulation with 10 ng ml -1 EGF for 10 min ( l ), while the non-stimulated cells only showed low basal signal ( k ). Under the same conditions phosphorylation of Akt could be observed ( n ), while no phosphorylation was visible in non-stimulated cells ( m ). In panel o , the phosphorylation of Syk is shown ( p : no primary antibodies). Detection of Her2 protein was possible as well and shown in panel q ( r : no primary antibodies). Expression of E-cadherin/beta-catenin interaction could be observed even in FFPE skin tissue ( s ), while no sig