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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-11402 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BNIP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Blockade of the CD93 pathway normalizes tumor vasculature to facilitate drug delivery and immunotherapy.
Proximity-dependent initiation of hybridization chain reaction.
Sun Y, Chen W, Torphy RJ, Yao S, Zhu G, Lin R, Lugano R, Miller EN, Fujiwara Y, Bian L, Zheng L, Anand S, Gao F, Zhang W, Ferrara SE, Goodspeed AE, Dimberg A, Wang XJ, Edil BH, Barnett CC, Schulick RD, Chen L, Zhu Y
Science translational medicine 2021 Jul 28;13(604)
Science translational medicine 2021 Jul 28;13(604)
Proximity-dependent initiation of hybridization chain reaction.
Koos B, Cane G, Grannas K, Löf L, Arngården L, Heldin J, Clausson CM, Klaesson A, Hirvonen MK, de Oliveira FM, Talibov VO, Pham NT, Auer M, Danielson UH, Haybaeck J, Kamali-Moghaddam M, Söderberg O
Nature communications 2015 Jun 12;6:7294
Nature communications 2015 Jun 12;6:7294
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BNIP3 using a BNIP3 polyclonal antibody (Product # PA5-11402) in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using a BNIP3 polyclonal antibody (Product # PA5-11402). HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with a BNIP3 polyclonal antibody (Product # PA5-11402) (1:500, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of mouse hepatocytes using a BNIP3 polyclonal antibody (Product # PA5-11402) at a dilution of 1:50. Freshly isolated mouse hepatocytes plated on coverslips (2x10^5 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a BNIP3 polyclonal antibody (Product # PA5-11402), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 In situ prox-HCR. ( a-d ) Technical controls for the E-cadherin/beta-catenin interaction. Strong membranous signal could be observed in HT29 cells when both primary antibodies were applied ( a ), while omitting either one of the primary antibodies ( b , c ) or both ( d ) did not yield visible signal. Phosphorylation of platelet-derived growth factor receptor-beta (PDGFR-beta) could be shown in BjHTert cells following stimulation with 100 ng ml -1 ( f ), while expression of phosphorylated receptor was low in non-stimulated cells ( e ). ProxHCR was used to visualize the induction of autophagy following starvation and incubation with CoCl 2 in Caco cells ( g-j ). Although untreated cells showed only low basal activity ( g ) of BCL2-BNIP3 interaction, a highly increased signal could be observed in treated cells ( h ). The same holds true for LC3-SQSTM1 interaction ( i , j ). The MEK-ERK interaction could be induced in A431 cells by stimulation with 10 ng ml -1 EGF for 10 min ( l ), while the non-stimulated cells only showed low basal signal ( k ). Under the same conditions phosphorylation of Akt could be observed ( n ), while no phosphorylation was visible in non-stimulated cells ( m ). In panel o , the phosphorylation of Syk is shown ( p : no primary antibodies). Detection of Her2 protein was possible as well and shown in panel q ( r : no primary antibodies). Expression of E-cadherin/beta-catenin interaction could be observed even in FFPE skin tissue ( s ), while no sig
