Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA052465 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA052465, RRID:AB_2681842
- Product name
- Anti-DNAJB13
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human DNAJB13, Gene description: DnaJ (Hsp40) homolog, subfamily B, member 13, Alternative Gene Names: RSPH16A, TSARG6, Validated applications: WB, IHC, Uniprot ID: P59910, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references A quantitative super-resolution imaging toolbox for diagnosis of motile ciliopathies
TTC12 Loss-of-Function Mutations Cause Primary Ciliary Dyskinesia and Unveil Distinct Dynein Assembly Mechanisms in Motile Cilia Versus Flagella
Liu Z, Nguyen Q, Guan Q, Albulescu A, Erdman L, Mahdaviyeh Y, Kang J, Ouyang H, Hegele R, Moraes T, Goldenberg A, Dell S, Mennella V
Science Translational Medicine 2020;12(535)
Science Translational Medicine 2020;12(535)
TTC12 Loss-of-Function Mutations Cause Primary Ciliary Dyskinesia and Unveil Distinct Dynein Assembly Mechanisms in Motile Cilia Versus Flagella
Thomas L, Bouhouche K, Whitfield M, Thouvenin G, Coste A, Louis B, Szymanski C, Bequignon E, Papon J, Castelli M, Lemullois M, Dhalluin X, Drouin-Garraud V, Montantin G, Tissier S, Duquesnoy P, Copin B, Dastot F, Couvet S, Barbotin A, Faucon C, Honore I, Maitre B, Beydon N, Tamalet A, Rives N, Koll F, Escudier E, Tassin A, Touré A, Mitchell V, Amselem S, Legendre M
The American Journal of Human Genetics 2020;106(2):153-169
The American Journal of Human Genetics 2020;106(2):153-169
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and DNAJB13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406985).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human fallopian tube and tonsil tissues using Anti-DNAJB13 antibody. Corresponding DNAJB13 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol