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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-36383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UCP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 33 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Active Vitamin D and Vitamin D Receptor Help Prevent High Glucose Induced Oxidative Stress of Renal Tubular Cells via AKT/UCP2 Signaling Pathway.
Zhu X, Wu S, Guo H
BioMed research international 2019;2019:9013904
BioMed research international 2019;2019:9013904
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UCP2 in Lane 1: HEK29T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate. Samples were incubated with UCP2 polyclonal antibody (Product # PA5-36383) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UCP2 in paraffin-embedded human colorectal cancer carcinoma tissue. Samples were incubated with UCP2 polyclonal antibody (Product # PA5-36383) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Protein and mRNA levels of HK2 cells analyzed by western blot and RT-PCR. (a), (b), and (c) show the protein levels of VDR, p-AKT, and UCP2 evaluated by western blot. (d) mRNA levels of VDR, AKT, and UCP2 measured by RT-PCR. Results are presented as the fold change in activity relative to normoxic cells. The data are expressed as the mean +- SD. *p
