11612-1-AP

antibody from Proteintech Group

Targeting: DCAF1 KIAA0800, MGC102804, VPRBP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11612-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11612-1-AP, RRID:AB_2216933

Product name

VPRBP antibody

Antibody type

Polyclonal

Description

KD/KO validated VPRBP antibody (Cat. #11612-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

CSN6 Promotes Pancreatic Cancer Progression and Gemcitabine Resistance via Antagonizing DCAF1-Mediated Ubiquitination of NPM1.

Zhang Y, Gao H, Tang A, Lyu H, Fan Z, Guo J, Wang Y, Yi H, Pan Q, Luo H, Qin B, Zhang B, Meng X, Liu Q, Lee MH
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(1):e10210

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DNA damage induced by HIV-1 Vpr triggers epigenetic remodeling and transcriptional programs to enhance virus transcription and latency reactivation.

Saladino N, Leavitt E, Wong HT, Ji JH, Ebrahimi D, Salamango DJ
PLoS biology 2026 Feb;24(2):e3003621

---

Molecular recording of cellular protein kinase activity with chemical labeling.

Sun DE, Ng SW, Zheng Y, Xie S, Schwan N, Breuer P, Hoffmann DC, Michel J, Azorin DD, Boonekamp KE, Winkler F, Wick W, Boutros M, Li Y, Johnsson K
Nature chemical biology 2025 Nov;21(11):1818-1827

---

RACK1 is required for normal B cell development and signaling but not RAG1 degradation.

Palmer VL, Schabla NM, Kumar V, Swanson PC
Journal of immunology (Baltimore, Md. : 1950) 2025 Nov 1;214(11):3003-3016

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Deciphering lentiviral Vpr/Vpx determinants required for HUSH and SAMHD1 antagonism highlights the molecular plasticity of these evolutionary conflicts.

Larrous P, Garnier C, Morel M, Martin MM, Zarrouk K, Maesen S, Matkovic R, Cimarelli A, Etienne L, Margottin-Goguet F
Journal of virology 2025 May 20;99(5):e0019825

---

STK39 inhibits antiviral immune response by inhibiting DCAF1-mediated PP2A degradation.

Zhang C, Xu P, Wang Y, Chen X, Pan Y, Ma Z, Wang C, Xu H, Zhou G, Zhu F, Xia H
Acta pharmaceutica Sinica. B 2025 Mar;15(3):1535-1551

---

HIV Vpr activates a nucleolar-specific ATR pathway to degrade the nucleolar stress sensor CCDC137.

Nisson KA, Patel RS, Delgado Y, Bouhaddou M, Etienne L, Fregoso OI
Nucleic acids research 2025 Jun 6;53(11)

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HIV-1 Vpr drives epigenetic remodeling to enhance virus transcription and latency reactivation.

Saladino N, Leavitt E, Wong HT, Ji JH, Ebrahimi D, Salamango DJ
bioRxiv : the preprint server for biology 2025 Jan 31;

---

VprBP regulates osteoclast differentiation via an epigenetic mechanism involving histone H2A phosphorylation.

Shin Y, Kim S, Choi TI, Kim CH, An W
Epigenetics & chromatin 2024 Nov 26;17(1):35

---

HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages.

Virgilio MC, Ramnani B, Chen T, Disbennett WM, Lubow J, Welch JD, Collins KL
Nature communications 2024 Jun 29;15(1):5514

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DOT1L/H3K79me2 represses HIV-1 reactivation via recruiting DCAF1.

Liang F, Jin J, Li Q, Duan J, Jiang A, Chen X, Geng H, Wu K, Yu F, Zhao X, Zhou Y, Hu D, Chen L
Cell reports 2024 Jul 23;43(7):114368

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HIV-1 Vpr-induced DNA damage activates NF-κB through ATM-NEMO independent of cell cycle arrest.

Sandoval C, Nisson K, Fregoso OI
bioRxiv : the preprint server for biology 2024 Jan 25;

---

HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages.

Virgilio MC, Ramnani B, Chen T, Disbennett WM, Lubow J, Welch JD, Collins KL
bioRxiv : the preprint server for biology 2024 Feb 27;

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Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer.

Ghate NB, Kim S, Shin Y, Kim J, Doche M, Valena S, Situ A, Kim S, Rhie SK, Lenz HJ, Ulmer TS, Mumenthaler SM, An W
Nature communications 2023 Apr 17;14(1):2140

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Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicating heterochromatin modulates methylcytosine oxidation.

Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, Ludwig AK, Carell T, Cardoso MC
Nature communications 2022 Sep 2;13(1):5173

---

Stabilization of SAMHD1 by NONO is crucial for Ara-C resistance in AML.

Zhang F, Sun J, Tang X, Liang Y, Jiao Q, Yu B, Dai Z, Yuan X, Li J, Yan J, Zhang Z, Fan S, Wang M, Hu H, Zhang C, Lv XB
Cell death & disease 2022 Jul 8;13(7):590

---

HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells.

Reuschl AK, Mesner D, Shivkumar M, Whelan MVX, Pallett LJ, Guerra-Assunção JA, Madansein R, Dullabh KJ, Sigal A, Thornhill JP, Herrera C, Fidler S, Noursadeghi M, Maini MK, Jolly C
Cell reports 2022 Apr 12;39(2):110650

---

The CRL4VPRBP(DCAF1) E3 ubiquitin ligase directs constitutive RAG1 degradation in a non-lymphoid cell line.

Schabla NM, Swanson PC
PloS one 2021;16(10):e0258683

---

VprBP directs epigenetic gene silencing through histone H2A phosphorylation in colon cancer.

Ghate NB, Kim S, Spiller E, Kim S, Shin Y, Rhie SK, Smbatyan G, Lenz HJ, Mumenthaler SM, An W
Molecular oncology 2021 Oct;15(10):2801-2817

---

Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages.

Murakami T, Matsuura R, Chutiwitoonchai N, Takei M, Aida Y
Viruses 2021 Nov 19;13(11)

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The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA.

Dar AA, Sawada K, Dybas JM, Moser EK, Lewis EL, Park E, Fazelinia H, Spruce LA, Ding H, Seeholzer SH, Oliver PM
PLoS biology 2021 Feb;19(2):e3001041

---

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes.

Dybas JM, Lum KK, Kulej K, Reyes ED, Lauman R, Charman M, Purman CE, Steinbock RT, Grams N, Price AM, Mendoza L, Garcia BA, Weitzman MD
mSystems 2021 Aug 31;6(4):101128msystems0046821

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DCAF1 regulates Treg senescence via the ROS axis during immunological aging.

Guo Z, Wang G, Wu B, Chou WC, Cheng L, Zhou C, Lou J, Wu D, Su L, Zheng J, Ting JP, Wan YY
The Journal of clinical investigation 2020 Nov 2;130(11):5893-5908

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The Potential of MLN3651 in Combination with Selumetinib as a Treatment for Merlin-Deficient Meningioma.

Lyons Rimmer J, Ercolano E, Baiz D, Makhija M, Berger A, Sells T, Stroud S, Hilton D, Adams CL, Hanemann CO
Cancers 2020 Jun 30;12(7)

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Vpr Targets TET2 for Degradation by CRL4(VprBP) E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.

Lv L, Wang Q, Xu Y, Tsao LC, Nakagawa T, Guo H, Su L, Xiong Y
Molecular cell 2018 Jun 7;70(5):961-970.e5

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HIV-1 Vpr hijacks EDD-DYRK2-DDB1(DCAF1) to disrupt centrosome homeostasis.

Hossain D, Ferreira Barbosa JA, Cohen ÉA, Tsang WY
The Journal of biological chemistry 2018 Jun 15;293(24):9448-9460

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Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, Diehl WE, Luban J
Nature microbiology 2018 Dec;3(12):1354-1361

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VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation.

Schabla NM, Perry GA, Palmer VL, Swanson PC
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):930-939

---

TET1 inhibits cell proliferation by inducing RASSF5 expression.

Li BT, Yu C, Xu Y, Liu SB, Fan HY, Pan WW
Oncotarget 2017 Oct 17;8(49):86395-86409

---

Primate lentiviruses use at least three alternative strategies to suppress NF-κB-mediated immune activation.

Hotter D, Krabbe T, Reith E, Gawanbacht A, Rahm N, Ayouba A, Van Driessche B, Van Lint C, Peeters M, Kirchhoff F, Sauter D
PLoS pathogens 2017 Aug;13(8):e1006598

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The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin.

Furukawa KT, Yamashita K, Sakurai N, Ohno S
Cell reports 2017 Aug 8;20(6):1435-1447

---

DCAF1 controls T-cell function via p53-dependent and -independent mechanisms.

Guo Z, Kong Q, Liu C, Zhang S, Zou L, Yan F, Whitmire JK, Xiong Y, Chen X, Wan YY
Nature communications 2016 Jan 5;7:10307

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Degradation of SAMHD1 by Vpx Is Independent of Uncoating.

Jáuregui P, Logue EC, Schultz ML, Fung S, Landau NR
Journal of virology 2015 May;89(10):5701-13

---

Characterization of the interactions between SIVrcm Vpx and red-capped mangabey SAMHD1.

Li J, Xu F, Hu S, Zhou J, Mei S, Zhao X, Cen S, Jin Q, Liang C, Guo F
The Biochemical journal 2015 Jun 1;468(2):303-13

---

Vpr Promotes Macrophage-Dependent HIV-1 Infection of CD4+ T Lymphocytes.

Collins DR, Lubow J, Lukic Z, Mashiba M, Collins KL
PLoS pathogens 2015 Jul;11(7):e1005054

---

Tumor suppressor protein Lgl mediates G1 cell cycle arrest at high cell density by forming an Lgl-VprBP-DDB1 complex.

Yamashita K, Ide M, Furukawa KT, Suzuki A, Hirano H, Ohno S
Molecular biology of the cell 2015 Jul 1;26(13):2426-38

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CRL4(VprBP) E3 ligase promotes monoubiquitylation and chromatin binding of TET dioxygenases.

Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, Fan HY, Chen X, Xiong Y
Molecular cell 2015 Jan 22;57(2):247-260

---

Cyclin L2 is a critical HIV dependency factor in macrophages that controls SAMHD1 abundance.

Kyei GB, Cheng X, Ramani R, Ratner L
Cell host & microbe 2015 Jan 14;17(1):98-106

---

CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation.

Yu C, Ji SY, Sha QQ, Sun QY, Fan HY
Nature communications 2015 Aug 18;6:8017

---

Defining the interactions and role of DCAF1/VPRBP in the DDB1-cullin4A E3 ubiquitin ligase complex engaged by HIV-1 Vpr to induce a G2 cell cycle arrest.

Gérard FC, Yang R, Romani B, Poisson A, Belzile JP, Rougeau N, Cohen ÉA
PloS one 2014;9(2):e89195

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A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Liu YZ, Jiang YY, Wang BS, Hao JJ, Shang L, Zhang TT, Cao J, Xu X, Zhan QM, Wang MR
Cancer cytopathology 2014 Nov;122(11):833-41

---

Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production.

Mashiba M, Collins DR, Terry VH, Collins KL
Cell host & microbe 2014 Dec 10;16(6):722-35

---

Ubiquitin E3 ligase CRL4(CDT2/DCAF2) as a potential chemotherapeutic target for ovarian surface epithelial cancer.

Pan WW, Zhou JJ, Yu C, Xu Y, Guo LJ, Zhang HY, Zhou D, Song FZ, Fan HY
The Journal of biological chemistry 2013 Oct 11;288(41):29680-91

---

Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP.

Wang BS, Liu YZ, Yang Y, Zhang Y, Hao JJ, Yang H, Wang XM, Zhang ZQ, Zhan QM, Wang MR
Clinical science (London, England : 1979) 2013 Feb;124(3):203-14

---

Linker Histone H1.2 cooperates with Cul4A and PAF1 to drive H4K31 ubiquitylation-mediated transactivation.

Kim K, Lee B, Kim J, Choi J, Kim JM, Xiong Y, Roeder RG, An W
Cell reports 2013 Dec 26;5(6):1690-703

---

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

Yu C, Zhang YL, Pan WW, Li XM, Wang ZW, Ge ZJ, Zhou JJ, Cang Y, Tong C, Sun QY, Fan HY
Science (New York, N.Y.) 2013 Dec 20;342(6165):1518-21

---

Differential effects of Vpr on single-cycle and spreading HIV-1 infections in CD4+ T-cells and dendritic cells.

de Silva S, Planelles V, Wu L
PloS one 2012;7(5):e35385

---

HIV-2 viral protein X (Vpx) ubiquitination is dispensable for ubiquitin ligase interaction and effects on macrophage infection.

McCulley A, Ratner L
Virology 2012 May 25;427(1):67-75

---

Vpr-binding protein antagonizes p53-mediated transcription via direct interaction with H3 tail.

Kim K, Heo K, Choi J, Jackson S, Kim H, Xiong Y, An W
Molecular and cellular biology 2012 Feb;32(4):783-96

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Vpx is critical for SIVmne infection of pigtail macaques.

Belshan M, Kimata JT, Brown C, Cheng X, McCulley A, Larsen A, Thippeshappa R, Hodara V, Giavedoni L, Hirsch V, Ratner L
Retrovirology 2012 Apr 24;9:32

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Vpx rescues HIV-1 transduction of dendritic cells from the antiviral state established by type 1 interferon.

Pertel T, Reinhard C, Luban J
Retrovirology 2011 Jun 22;8:49

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP(VPRBP antibody) at dilution of 1:500

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The VPRBP antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VPRBP. This antibody recognizes human,mouse,rat antigen. The VPRBP antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

11612-1-AP.pdf

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