11612-1-AP

antibody from Invitrogen Antibodies

Targeting: DCAF1 KIAA0800, MGC102804, VPRBP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11612-1-AP

Provider

Invitrogen Antibodies

Product name

VPRBP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: WSQPLSALW GMKSVFDMKH SFTEDHYVEF SKHSQDRVIG TKGDIAHIYD IQTGNKLLTL FNPDLANNYK RNCATFNPTD DLVLNDGVLW DVRSAQAIHK FDKFNMNISG VFHPNGLEVI INTEIWDLRT FHLLHTVPAL DQCRVVFNHT GTVMYGAMLQ ADDEDDLMEE RMKSPFGSSF RTFNATDYKP IATIDVKRNI FDLCTDTKDC YLAVIENQGS MDALNMDTVC RLYEVGRQRL AEDEDEEEDQ EEEEQEEEDD DEDDDDTDDL DELDTDQLLE AELEEDDNNE NAGEDGDNDF SPSDEELANL LEEGEDGEDE DSDADEEVEL ILGDTDSSDN SDLEDDIILS LNE (707-1058 aa encoded by B C022792)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 11612-1-AP (VPRBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 11612-1-AP (VPRBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 11612-1-AP (VPRBP antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP ( VPRBP Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP ( VPRBP Antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug VPRBP antibody (11612-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug.